Detection of bovine parainfluenza type 3 virus in Iraq Aida Bara Allawe and Anton Sabri Al-bana

Abstract

This study is considered the first study in our country to isolate bovine parainfluenza
type 3 virus (BPIV-3) from naturally infected calves. BPIV-3 virus was isolated from nasal
swabs collected from calves with respiratory disease. The virus was isolated and titrated in
secondary embryonic bovine kidney (SEBK) cell culture; other cell cultures (secondary sheep
testis and chicken embryos fibroblast cell cultures) were used for propagation of isolated
virus. Examination of infected cell culture revealed giant cell formation and intracytoplasmic
inclusions. The isolated virus agglutinated guinea pig’s erythrocytes and produces clear
heamadsorption of guinea pig’s erythrocyte in infected SEBK cell culture. Virus titer was
2×102
TCID50/0.1ml in the sixth passage and became 2×104.5TCID 50/0.1ml in the ninth
passage. The new isolated virus was identified as BPIV-3 by heamagglutination inhibition
test, serum neutralization test and indirect immunoflurescent technique, by using specific
monoclonal and polyclonal antibody. The virus was sensitive to ether. High titer of BPIV-3
antibodies was detected in sera of infected animals during convalescent period by ELISA test