Isolation and detection of rotavirus by Enzyme linked Immune assay in fecal specimens of buffalo calves
Article Sidebar
Main Article Content
Abstract
A total of 50 fecal samples were collected from buffalo calves ages between 3 days to 4 months, in middle area of Iraq (Baghdad, Salahaldden, Babylon, Diyala, and Wasit) between July 2016 to June 2017. All samples were examined by Immuno-chromatographic rapid tests for detection of buffalo rotavirus, twenty samples were positive. Enzyme-Linked Immunosorbent Assay. test was also used and revealed only 8 positive samples, the later samples were used for viral isolation on fetal bovine kidney cell culture for detection the cytopathogenic effects of the virus. The cytopathogenic effects of virus were induced rounded cells and gaps; as a result of rupture of these infected cells by rotavirus. Moreover Enzyme-Linked Immunosorbent Assay test used again for detection of rotavirus cytopathogenic effect in fetal bovine kidney cell culture for more confirmation. The TCID 50 was estimated for rotavirus on fetal bovine kidney cells 104.5 virus/0.05 ml
Received: 7/5/ 2018
Accepted: 3/6/ 2018
Publishing:31 /1/2019
Downloads
Article Details
How to Cite
References
Dennehy, P.H. (2000). "Transmission of rotavirus and other enteric pathogens in the home". Pediatr. Infect. Dis. J.,19(10 ): S103–105
Anonymous, (2014). The Cattle Site –Cattle Health, Welfare and Diseases News. 5m Publishing, Benchmark House, 8 Smithy Wood Drive, Sheffield, S35 1QN, England. –accessed on 22 Feb 2017; http://www.thecattlesite.com/diseaseinfo/234/rotaviraldiarrhoea/#sthash.q8QVqU6n.dpuf.
Anthony, I.D.;Dayal, S.B.; Bellamy, A.R. and Berriman. J.A. (1991). Rotavirus spike structure and polypeptide composition. J. Virol.,
:4334-4340.
Lyoo. Y.S. (1995). Expression and characterization of bovine rotavirus proteins in the baculovirus system. Dissertation. Department of Veterinary microbiology and Preventive medicine. Iowa State University.
Patton, J.T. (2012). Rotavirus Diversity and Evolution in the Post-Vaccine World, discovery. Institution: Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of HealthAddress: 50 Center Drive, Bethesda,
Maryland, 20892, United States.
Buser, J.; Risch, L.; Rutz, T.; Manang, S. and Munzinger, J. (2001). Comparison of rotavirus latex agglutination test with two rapid
immunochromatographic test devices for detection of rotavirus in human feces. J. Clin. Microbiology Infect Dis., 20: 295-296.
Altindis, M.; Yavru, S.; Simsek, A.; Ozkul, A.; Ceri, A. and Koc, H. (2004). Rotavirus infection in children with acute diarrhea as detected by latex agglutination, ELISA and polyacrylamide gel electrophoresis. Indian. 41(6):590-594.
AL-Rawi, A.S. (2013). Serological and Molecular identification of foot and Mouth disease from different area in Iraq. Ph.D. dissertation. Department of microbiology. College of Veterinary Medicine, University of Baghdad.
Villages, P. and Purchase, H.G. (1989). A laboratory manual for the isolation and identification avian pathogens. 3th
ed. university of Pennsylvania. New Bolton center Kennett, PA. Pp :187-188.
Hassan.H.A.; Kshash, Q.H. andMansur, K.A. (2014). Detection of bovine rotavirus in diarrheic calves by using rapid test in some Mid-Euphrates provinces. AL-Qadisiya J of Vet. Med. Sci. . 13(2):20-26.
Guandalini, S. and Vaziri, H. (2011). Diarrhea: Diagnostic and Therapeutic Advances. Humana Press, Springer Science Business Media. 233 Spring Street. New York. USA.
Abed, M.N. (2017). Genotyping of rotavirus isolated from infected neonatal calves with acute gastroenteritis in different area of Iraq
Thesis, Department of microbiology, College of Veterinary Medicine, University of Baghdad.
Al-Gburi,R.S.J. (2013).Role of Poultry Star and Alcoholic Myristica fragrans extract on calf rotavirus diarrhea.Msc Thesis.Department of microbiology. College of Veterinary Medicine. University of Baghdad.
Curtis, Jeanette and Caroline Rea (2007)."Viral culture". WebMD. Retrieved 2009-09-09. http://www.webmd.com/hw/health_gui
de_atoz/stv5474.asp
Bartlett, J.M.S. and Stirling, D. (2003). "A Short history of the polymerase Chain Reaction". PCR Protocols. Methods in Molecular Biology. 226 (2nd ed.). pp: 3–6.
Jumaa, R.S. (2017). Antiviral effect of myristica fragrans on calf rotavirus (in vitro). International Journal of Science and Nature,
(1) : 1-6
Linda J.S.; Redman, D.R.; Smith, K.L. and Theil, K.W. (1983).Passive immunity to bovine rotavirus in newborn calves fed colostrum
supplements from immunized or nonimmunized cows. Infection and immunity, 41(3): 1118-1131
Linda J.S.; Rosen, B.I.; Kang, S.Y. and Miller, K.L. (1988). Cell culture propagation of rotaviruses,Journal of Tissue Culture Methods ,11(3):147 –156.
Aiyegbo, M.S.; Benjamin, A.E.; Spiller, W.; William, S.D.R;Kim, R.; Lee, D.E.; Liu, T.; Li,S.; Stewart, P.L. and Crowe, JE. (2014). Differential
accessibility of a rotavirus VP6 epitope in trimers comprising Type I, II, or III channels as revealed by binding of a human rotavirus VP6-specific antibody. J. Virol., 88(1): 469–476.
Kaufmann, S.H. and Kabilitz, D. (2002). Method in microbiology Vol.32:immunology of infection. Academic press.
ISBN 0-12-521532-0.
Arnold, M.; Paton.J.T. and sarah M. Mc Donald,. 2009. Culturing, storage, and quantification of rotavirus. Curr
Protoc Microbiol. CHAPTER: Unit–15C.3.
Ward, R.L.; Celemens, J.D.;Sack, D.A.; Knowlton, D.R.; Mcneal, M.M.; Huda, N.; Ahmed, F.; Rao, M. and
Sciff, G.M. (1991).Culture adaptation and characterization of group A rotavirus causing diarrheal illnesses in
Bangaladish from 19885-1986. j of Clinical Microbiology, 29(9): 1915-1922.