SURVIVAL OF DIFFERENT BACTERIAL SPECIES ON CEMENT AND PORCELAIN SURFACES. AT LOW TEMPERATURE.
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Abstract
Fourteen different bacterial species were used in a study of their abilities to survive on cement and porcelain surfaces. A dose of 5x10 cfu/drop (50 ul) for each species was used to contaminate each surface. The bacteria used included: Bacillus subtilis, Brucella abortus, Coraynebacterium pyogenes, E.coli, Klebsiella pnemmoniae, Listeria monocytogenes, Pasteurella multocida, Proteus vulgaris, Pseudomonas aergenosa, Salmonella typhi, Staphylococus aureus, Staph. Epidermidis, Streptococcus equi & Strept. Zooepidemicus. After 7, 14 and 21 days of storage at 4°C, subsamples were cultured onto different types of media, and after 24-48 hr. of incubation at 37°C, growth or no growth were scored for each type of bacteria used.
Results showed that B. subtilis, Staph.epidermidis & Strept. zooepdemicus were recovered from all surfaces up till 21-day storage. E. coli, Pr.vulgaris, Staph. aureus & Strept.equi were recovered only from Porcelaim surfaces throughout the whole experiment, whereas Past. multocida was recovered at all times from cement surfaces only. Br. abortus was recovered only from cement surfaces after 7 & 14 days of storage, but was never isolated from contaminated Porcelain surfaces. Kl. pneumoniae was isolated from Porcelain surfaces at all times, but only after 7-day- storage from cement surfaces. On the other hand, L. monocytogenes was never recovered from any surface at any time of storage.
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Absolom D.R.; F.V. Lamberti; Z. Policova; W. Zingg, C.L. vanoss A.W. Naumann (1983). Surface thermodynamics of bacterial adhesion. Appl. Env. Mirobiol. 46:90-97.
Al-obaidi A.S.R. (1995). Survival of different types of bacteria on different inanimate surfaces stored under different conditions: (1) Survival of E.coli on different solid surfaces stored at two different temperatures. J.Iraqi. Vet. Med.Ass. Vol,19 (under press).
Cowan S.T (1997). Cowan & Steel's manual for identification of medical daoteria. By Cowan S.T. (Ed.), 7th ed. Cambridge Univ. Press, U.K.
Fletcher M. (1977). The effects of culture-concentration and age time and temperature and bacterial attachment to polystyrene. Can. J. Microbiol. 23:1-6.
Harden V.P. & J.O. Harris (1953). The isoelectric point of bacterial cells. J. Bacteriol. 65:198-202.
Mc Eldowney S. & M. Fletcher (1986). Variability of the influence of physicochemical factors affecting bacterial adhesion to polystyrene substrata. Appl. Env. Microbiol. 52:460-465.
Rose A.H. (1976). The Environment; In: Chemical Microbiology, An introduction to microbial physiology, By A.H. Rose (Ed.), 3rd. ed. Butterworths, London. pp.93-149.
Rutter P.R. & B. Vincent (1980). The adhesion of micro-organisms to surfaces; physicochemical aspects. pp.79-91; In: R.C. Berkeley, J.M. Lynch, J. Melling, P.R. Rutter & B. Vincent. (Ed.). Microbial adhesion to surfaces. Halstead Press, New York.
Woods D.R.; D.C. Stress; W.G. Johanson; V.K. Berry & J.A. Bess. (1980) Role of pili of Pseudomonas aeruginosa to mammalian buccal epithelial cells. Infec. Immun. 29:1146 1151.