RADIOIMMUNOASSAY FOR DETECTION OF ANTI-RINDERPEST ANTIBODIES IN CATTLES

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HM. ALI
G.S. MOHAMMAD
HM. ABDULKARIM

Abstract

Liquid Phase Radioimmunoassay (RIA) was developed to detect and measure anti-rinderpest immunoglobulins in field animals sera, within two hours. Rinderpest virus adapted on Vero cell line culture and antigen purified by treatment with Triton-Gentron 13 Butanol, and, labeled with I isotope, using chloramin T iodination method. 


Comparative studies for detecting anti-rinderpest immunoglobulin in 80 calves sera samples, using the developed assay in parallel with virus neutralization test (VNT). The study showed 58.75 % agreement between the two methods. However, 71 % of seven months old, non-vaccinated calves showed anti rinderpest antibodies in their sera, also 81 % of 10 months old vaccinated calves were developed antibodies in their blood. 


These results demonstrate the development of sensitive, specific and rapid quantitative / qualitative radioimmunoassay, necessary for screening the development of immunity against rinderpest in cattle.

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RADIOIMMUNOASSAY FOR DETECTION OF ANTI-RINDERPEST ANTIBODIES IN CATTLES . (2022). The Iraqi Journal of Veterinary Medicine, 21(1), 85-99. https://doi.org/10.30539/ijvm.v21i1.1248
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How to Cite

RADIOIMMUNOASSAY FOR DETECTION OF ANTI-RINDERPEST ANTIBODIES IN CATTLES . (2022). The Iraqi Journal of Veterinary Medicine, 21(1), 85-99. https://doi.org/10.30539/ijvm.v21i1.1248

References

Rossiter,P.B.,Jossett,D.M. and Holmes, P. (1981). Micro ELISA test for detecting antibodies to RPV antigens. Trop.

Animal Health and Prod. 13 : 113-116.

Anderson, J.Rowe,L.W. et al (1982). An ELISA for detection of IgG, IgA and IgM antibodies to RPV in experimentally

infected cattle. Res. Vet. Sci. 32 : 242-247.

Sharma B.,Joshi, R.C. et al (1983). Evaluation of ELISA in Rinderpest. Indian J. Anim. Sci. 53 : 1292-1293.

Anderson, J., Rowe,L.W., Taylor, W.P. (1983). Use of ELISA for detection of antibodies to RPV in epidemiological

surveys. Res. Vet. Sci. 34 : 77-81.

Ali,H.M. (1987). Materno-foetal transfer of immunity against influenza virus infection in guinea pigs. PhD thesis,

submitted to University of Newcastle upon Tyne, U.K.

Nawroz,AH. (1984). Assay of Rinderpesť antibodies by an improved micro-enzyme linked immuno sorbent assay (ELISA) technique. M.Sc. Thesis submitted to the University of Agricultural sciences, Banglore, India.

Brown, R.D. (1958). Rinderpest immunity in calves-areview. Bull. Epiz Dis. Afr. 6:127-133.

Rawe, L.W.,Zwert,D., and Konwen hoven, P. (1968). A

comparison of HI and NT in the assay of rinderpest antibodies in cattle. Bull. Epiz. Dis. Afr. 15 : 301-306.

Provost, A., and Borredon, C. (1968). On rinderpest virus: 2 Rinderpest haemagglubinin, antigenic relationship rinderpest and

Measles virus. Vevue, Elev., Med. Vet. Pays., Trop. 21 : 33-48.

Shankaralingam, S.,Masalamony, R.P., Jayaraman, M.S., et al (1979). Evaluation of Rinderpest by MHI test and serum

neutralization test, Cheiron; 8, 240-246.

Jayeraman, M.S., Richard, M.P., Nachimuthu, K. and Padmanban, V.D., (1979). The usefulness of MHI test in detecting rinderpest antibodies in cattle and sheep after vaccination of tissue culture rinderpest vaccine, India Vet. J., 5b: 816-820.