RADIOIMMUNOASSAY FOR DETECTION OF ANTI-RINDERPEST ANTIBODIES IN CATTLES

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1997.1.8
Published: Jan 11, 2022
DOI: https://doi.org/10.30539/ijvm.v21i1.1248
Keywords:
rinderpest, radioimmunoassay, cattles

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HM. ALI
Dept. of Microbiology - College of Med. - Uni. of Baghdad.
G.S. MOHAMMAD
Dept. of Microbiology - College of Agricul. – Uni. of Baghdad
HM. ABDULKARIM
Nuclear Research Center - INEO- Baghdad.

Abstract

Liquid Phase Radioimmunoassay (RIA) was developed to detect and measure anti-rinderpest immunoglobulins in field animals sera, within two hours. Rinderpest virus adapted on Vero cell line culture and antigen purified by treatment with Triton-Gentron 13 Butanol, and, labeled with I isotope, using chloramin T iodination method. 


Comparative studies for detecting anti-rinderpest immunoglobulin in 80 calves sera samples, using the developed assay in parallel with virus neutralization test (VNT). The study showed 58.75 % agreement between the two methods. However, 71 % of seven months old, non-vaccinated calves showed anti rinderpest antibodies in their sera, also 81 % of 10 months old vaccinated calves were developed antibodies in their blood. 


These results demonstrate the development of sensitive, specific and rapid quantitative / qualitative radioimmunoassay, necessary for screening the development of immunity against rinderpest in cattle.

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RADIOIMMUNOASSAY FOR DETECTION OF ANTI-RINDERPEST ANTIBODIES IN CATTLES . (2022). The Iraqi Journal of Veterinary Medicine, 21(1), 85-99. https://doi.org/10.30539/ijvm.v21i1.1248
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Vol. 21 No. 1 (1997): Iraqi J. Vet. Med.
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Articles
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This work is licensed under a Creative Commons Attribution 4.0 International License.

 

 

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RADIOIMMUNOASSAY FOR DETECTION OF ANTI-RINDERPEST ANTIBODIES IN CATTLES . (2022). The Iraqi Journal of Veterinary Medicine, 21(1), 85-99. https://doi.org/10.30539/ijvm.v21i1.1248
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