Comparison of Histomorphometric Study of Chromaffin Cells in Adult Males Squirrel (Sciurusanomalus) and Hamster (Mesocricetusauratus)
The adrenal glands are endocrine glands that produce a diversity of hormones comprising of adrenaline, the aldosterone and cortisol. The present study aimed at investigation of the histomorphometric features of chromaffin cells. There were two types of chromaffin cells. In squirrel, the first type was columnar in shape and brownish in color contained spherical nucleus located at the base of cells, which represented the epinephrine secreting cells, and the second type was polygonal in shape and light brownish in color contained spherical nucleus located in the center of cells, which represented the norepinephrine secreting cells. The adrenal medulla of hamster consists almost entirely of columnar or polyhedral chromaffin cells forming clusters and anastomosing cords separated by sinusoids, giving a strong reaction with methylene eosin stain more than that seen in squirrel. The statistical analysis showed that the means diameter of epinephrine cells and norepinephrine cells in the right adrenal gland in squirrel were lesser than those of hamster significantly at P<0.05, but in the left adrenal gland in squirrel the means were greater than those of hamster significantly at P<0.05. In conclusion, the present findings showed the reaction of chromaffin cells of hamster with methylene –eosin stain to be stronger than with hematoxylin-eosin stain, while the opposite was true in case of the squirrel.
Al-Rubaie N. Anatomical and histological study of adrenal gland in Iraqi buffalo (Bubalus bubalis) with referring to the seasonal changes [Thesis]. Baghdad: Iraq. University of Baghdad; 2007.
Abbas L, Shakir M. Histomorphological and histochemical study of adrenal gland in adult male of guinea pigs (Cavia porcellus). The Iraqi. J. Vet. Med.2019: 43(1):59–66p.
Yılmaz S, Girgin A. Light and electron microscopic observations on the structure of the porcupine (Hystrix cristata) adrenal gland. Vet. Arhiv. 2005:75 (3): 265-272.
Stevens A, Lowe JS. Histology picture tests paperback. London: Gower medical pub; 1992 .260-263.
Dellman HD, Brown EM. Endocrinology textbook of veterinary histology. 3rd ed. Philadelphia: Lea and Febiger;1987. 361-381p.
6-Sheikhian A, Saadatfar Z, Mohammad AA. A histological study of adrenal gland in guinea pig and hamster, Comp Clin Pathol., 2014:111:782-789.
Enemali FU. Anatomical studies on thyroid, parathyroid and adrenal gland of the African giant rat (Cricetomys gambianus Water House-1849) [Thesis] Zaria: Nigeria, Bello University, 2014.
8- Bacha WJ, Bacha LM. Color atlas of veterinary histology. 2nd ed. USA, New York: John-Wiley-Sons-Inc; 2012; 191–200 p.
Al-sharoot HA. Histological study of the adrenal gland in guinea pig (Cavia porcellus). Int J Adv Res. 2014: 2(9): 755-760.
Mughal IA, Qureshi AS, Tahir MS. Some histological observations on postnatal growth of rat adrenal gland with advancing age. Int. J. Agri. Biol. 2005: 6(2):413-417.
Baiomy WK, Anas A, Ghaly M, Moustafa AM, Mohamed A W. Quantitative and histochemical study on the adrenal medulla in post-natal period of albino rat. Egypt J. Hosp Med. 2009: (36) 468-482.
Ezumi W, Amrah SS, Farid CG, Mohsin SJ. Morphological characteristics of the adrenals of Rattus norvegicus: a revisit by scanning electron microscopy. Annals of microscopy J. 200; 62-69.
Goodarzi N, Akbari G. Histomorphometry of adrenal gland of squirrel Sciurus anomalus. J Vet Res.2016: 20(11):691-697.
Sisson and Grossman. The anatomy of domestic animals revised by Grossman, J.D. 5th ed. WB. Saunders company: Philadelphia;1979: 573-580p.
Santos C, Viana DC, Bertassoli BM, Vasconcelosc BG, Liveira DM, Rici EG. Adrenal glands of Spix’s yellow-toothed cavy (Galeaspixii, Wagler, 1831): morphological and morphometric aspects. Braz. J. Biol. 2016: 76(3):.645-655.
Ribes AC, Mohamed F, Dominguez S, Delgado M, Scardapane L, Guzman J. Probable effect of photoperiod on seasonal variation in the nuclear volume of the adrenal cortex of viscacha (Lagostomus maximus maximus). Braz J Med Biol Res.1999:32(9):1115-1120.
Received: 2 December,2020
Accepted: 11 January 2021
Published: 28 Jun 2021