Evaluation of interleukins (2, 6 and 8) in immunized white rats by Salmonella enterica subspecies typhimurium and Cryptococcus neoformans antigens

Abstract

     This study was designed to evaluate the levels of interleukins (2, 6 and 8) in immunized white  rats by killed whole cell antigens of Salmonella enterica subspecies typhimurium and sonicated Cryptococcus neoformans, and using  ELISA in day 10, 20, 30, 40 and 50 after immunization one hundred white rats of both sexes divided into five groups (20 rats for each). The first group was immunized by killed whole cell antigens of Salmonella enterica subspecies typhimurium (9× 10⁸ CFU /ml) and sonicated Cryptococcus neoformans (1000 µg/ml), The second was immunized by killed whole cell antigens of Salmonella enterica subspecies typhimurium (9× 10⁸ CFU /ml) and sonicated Cryptococcus neoformans (500 µg/ml). The third was immunized by killed whole cell antigens of Salmonella enterica subspecies typhimurium (9× 10⁸ CFU /ml) as positive control group, The fourth was injected 1 ml of phosphate buffer saline (pH 7.2) as control negative group and fifth was immunized by sonicated antigens of Cryptococcus neoformans (1000 µg/ml). The results of IL-2 showed significant differences (P<0.05) between the 1^st, 2^nd and 3^th groups compared with 4^th and 5^th group, while there was no significant difference (P≥0.05) between 4^th and 5^th groups. Also IL-6 showed that there were significant differences (P<0.05) between the 1^st, 2^nd and 3^rd groups in comparison with 4^th group, while there was no significant difference (P≥0.05) between the 1^st, 2^nd, 3^rd groups and 5^th group. In the IL-8 showed that there was a significant difference (P<0.01) between the1^st and 2^nd groups and between 3^rd group and 1^st and 2^nd groups without significant difference (P≥0.05), also between the 1^st, 2^nd and 3^rd groups and 4^th group significant difference (P<0.05) and with a significant difference (P<0.01) between 5^th group and all other groups (1^st, 2^nd, 3^rd and 4^th).